1990's Teenage Mutant Ninja Turtles made one of its studio backers nervous with its darker tone, but that proved to be a major selling point for the film.
Donatello, Raphael, Michelangelo, and Leonardo looking distraught in Teenage Mutant Ninja Turtles (1990) - New Line Cinema Before Jesus, there was Leonardo, Michelangelo, Donatello, and Raphael.