Grow the MEF cultures to confluency, collect the cells and freeze (one vial/flask) as described in Steps 16–20 These are passage-zero MEFs. Split each flask (from Step 3) 1:5 to generate 25 ...

Primary human brain microvascular ECs (HBMVECs; Applied Cell Biology Research Institute, Kirkland, WA, USA) and immortalized primary HBMVECs (hCMEC/D3) were used in experiments with consistent and ...

Working very much at the sped-up end of the data spectrum is data streaming platform company Confluent, Inc. Now working to enable new methods of creating data streams for data pipelines ...