No, Plo Koon does not appear in Star Wars: The Acolyte. However, a member of his species, the Kel Dor, does appear in episode 4 as part of the Jedi expedition to Khofar.

Master Plo Koon of the Jedi Council had an orange lightsaber in Legends comics with ... although it has been replaced with green in canon. Darth Bane, founder of the Sith Rule of Two, used an ...