Recent advances in single-cell RNA sequencing of bacteria: …
May 1, 2025 · This review surveys the recent developments in bacterial scRNA-seq techniques, highlighting the technical challenges, methodological innovations, and emerging applications …
Transcriptomic profiling of individual bacteria by MATQ-seq
Apr 9, 2025 · Here we provide a step-by-step protocol covering the entire process of generating single-bacteria transcriptomes, including experimental and computational analysis.
Identification of intracellular bacteria from multiple single-cell RNA ...
We developed a computational pipeline (CSI-Microbes) for identifying microbial reads from single-cell RNA sequencing (scRNA-seq) data and for analyzing differential abundance of taxa.
Methodologies for bacterial ribonuclease characterization using RNA-seq …
Over the past decade, researchers have established many RNA-seq-based methodologies to study bacterial RNases, providing large-scale data and improving our understanding of the …
Advancing massive transcriptional profiling of single bacteria
Feb 27, 2023 · Single-bacteria RNA sequencing is becoming a transformative technology for elucidating bacterial diversity in ecology, microbial communities, and pathogenicity.
Bacterial single-cell RNA sequencing captures biofilm …
Nov 24, 2024 · Here, Korshoj & Kielian present an optimized bacterial single-cell RNA sequencing method and use it to study Staphylococcus aureus diversity during biofilm growth and …
Bacteria: recording gene activity more efficiently | RNA-Seq Blog
Researchers at Julius-Maximilians-Universität (JMU) Würzburg and the Helmholtz Institute for RNA-based Infection Research (HIRI) in Würzburg developed an innovative variant of …
Ushering in a new era of single-cell transcriptomics in bacteria
Sep 21, 2022 · Transcriptome analysis of individual cells by single-cell RNA-seq (scRNA-seq) has become routine for eukaryotic tissues, even being applied to whole multicellular organisms.
Bacterial droplet-based single-cell RNA-seq reveals antibiotic ...
Feb 16, 2023 · We introduce BacDrop, a highly scalable technology for bacterial single-cell RNA sequencing that has overcome many challenges hindering the development of scRNA-seq in …
Bacterial RNA Sequencing: Methodologies and Insights
As we embark on this comprehensive examination, it's essential to highlight the methodologies used in bacterial RNA sequencing, the obstacles encountered, and the implications these …